Nathan Shock Center:

The University of Oklahoma Health Sciences Center 

Oklahoma City, Oklahoma
Partner 4

Center Director

Arlan Richardson, PhD

Arlan Richardson, PhD

Email

Contact Details

Website |

ROCA/Geriatric Medicine
975 NE 10th Street/SLY-BRC 1303
Oklahoma City, OK 73104

Program Enhancement

Services

  • Provide both intellectual and organizational leadership in developing a research emphasis in Geroscience in Oklahoma City as well as the State of Oklahoma
  • Develop a Oklahoma Geroscience Consortium to bring together all faculty in Oklahoma who are interested in aging and age-related diseases

Core Leaders

Arlan Richardson, PhD

Arlan Richardson, PhD

Email
Holly Van Remmen, PhD

Holly Van Remmen, PhD

Email

Research Development

Services

  • Provides investigators at institutions throughout the United States conducting geroscience related research with pilot funding. These funds can be used to access the resources of the Oklahoma Nathan Shock Center at no cost. These NIA sponsored funds are typically reserved for junior faculty members at the early stages of their career although more senior investigators that are new to the aging field also may be eligible for funding

Core Leader

William Sonntag, PhD

William Sonntag, PhD

Email

Multiplexing Protein Quantification

Services

  • Uses LC-tandem MS with selected reaction monitoring (SRM) to measure protein abundance in tissue samples from rodents as well as invertebrates and other animals used in geroscience research
  • Panels of proteins (20 to 30) in the following pathways are available for rats and mice: antioxidant proteins, β-oxidation, glycolysis, TCA-cycle, and various mitochondrial proteins
  • Assays for proteins involved in other pathways can be designed and validated for rodents and humans
  • Panels of proteins for invertebrates (yeast, C. elegans, Drosophila) and exceptionally long-lived species (e.g., naked mole rats, bats, birds, etc.) can be developed

Core Leader

Michael Kinter, PhD

Michael Kinter, PhD

Email

Targeted DNA Methylation & Mitochondrial Heteroplasmy

Services

  • A genome-wide, base-specific analysis of 5mC levels in all gene promoters and all of the non-repeat region CpG islands, shores, and shelves of the human, mouse, or rat genome using Bisulfite Oligonucleotide Capture Sequencing assay
  • Bisulfite Amplicon Sequencing gives investigators DNA methylation in the promoters or in other region of interest (a few hundred bases to a few kilobases) of a specific gene from any species
  • Whole mitochondrial genome sequencing for human, mouse, and rat (as well as other animal models, including invertebrates) to identify heteroplasmic variants
  • Absolute quantification of mitochondria DNA copy number in human, mouse, rat, and fly samples

Core Leader

Willard "Bill" Freeman, PhD

Willard "Bill" Freeman, PhD

Email

Integrative Redox Biology

Services

  • Measure of redox status, e.g., GSH/GSSG, NADPH/NADP+, NADH/NAD+
  • Mitochondrial function analysis in cells, tissues and isolated mitochondria
  • Measures of oxidative damage, e.g., F2-isoprostanes, 8-oxo-deoxy guanosine, and protein carbonyls

Core Leader

Holly Van Remmen, PhD

Holly Van Remmen, PhD

Email

Discovery Bioinformatics

Services

  • Provides support in statistics and bioinformatics for data analysis, including prioritization of Y2H PPI studies
  • Processing and analysis of ChIP-seq, RNA-seq and microarray experimental data
  • Text-mining analysis to identify published commonalities, how two things are connected, or infer novel connections
  • Transcriptional network analysis to predict gene or ncRNA function, even when no literature exists on the transcript

Core Leader

Jonathan D. Wren, PhD

Jonathan D. Wren, PhD

Email