Oklahoma Nathan Shock Center
Oklahoma City, OK
Specialized Core Areas
Administrative/Program Enrichment Core
Services
- Provides intellectual and organizational leadership and evaluates the Center’s impact on aging and geroscience research programs locally and nationally, including through its Internal and External Advisory Committees
- Oversees, manages and coordinates the execution of all NSC programs and activities, monitors progress, and ensures coordination between all Cores · Oversees the activities of the Geroscience Biorepository and the Geroscience Toolbox
- Organizes and runs the Geroscience Webinar Series
- Oversees the Geroinformatics Resource · Organizes the annual Oklahoma Nathan Shock Center Geroscience Symposium to discuss advances in the biology of aging and geroscience, promote collaborations, expand research networks, learn about funding priorities and opportunities, and serve as a forum for NSC Pilot Grant and K-series grant awardees, as well as early career investigators participating in our Pilot Programs and in Oklahoma NSC Mentorship Program of the Research Development Core
Research Development Core
Services
- Provides funded access to cutting-edge, transformative technologies to investigators in the aging and geroscience fields or starting a research program in these fields through General Call Pilot Awards, Translational Pilot Awards and Gene Discovery Pilot Awards
- Provides mentoring, technical, and collaborative resources to promote the development of, or the transition to, an independent career in the biology of aging/geroscience through its Mentorship Program, K/F-Troop Workshops, and Grant Bootcamp Program
- Ensures dissemination of knowledge while promoting participation of all investigators in aging/geroscience to benefit from core services and other research and career advancement activities of the Oklahoma NSC, and disseminates knowledge of aging/geroscience and the NIA NSC Program to the general public, both through its Outreach and Communications component
Multiplexing Protein Analysis Core
Services
- LC-tandem MS with selective reaction monitoring (SRM) to measure protein panel abundance (antioxidant and mitochondrial proteins, proteins involved in β-oxidation, glycolysis, TCA cycle, branched-chain amino acid catabolism, others) in tissues and cells used in geroscience research
- Development of new assays for additional proteins/protein panels as requested by investigators in any model system
- Large-scale quantitative proteomics studies using data independent acquisition (DIA)
- Measures of protein turnover using deuterium oxide labeling
Genomic Sciences Core
Services
- Genome-wide, base-specific analysis of DNA modification levels by nanopore sequencing
- Whole mitochondrial genome sequencing for human, mouse, and rat (as well as other animal models, including invertebrates) to identify heteroplasmic variants
- Absolute quantification of mitochondrial DNA copy number in human, mouse, and rat samples by digital PCR
Core Leader
Willard "Bill" Freeman, PhD
EmailGeroscience Redox Biology Core
Services
- Assays of redox status, e.g., GSH/GSSG, NADPH/NADP+, NADH/NAD+
- Quantification of TCA intermediates (other metabolites available upon request)
- Assays of oxidative damage, e.g., F2-isoprostanes, and protein carbonyls
- Mitochondrial function in cells and tissues (fresh or frozen)
- Free radical measurement by EPR spin-trapping
Core Leader
Kenneth Humphries, PhD
EmailCRISPR Whole Genome Screening Core
Services
- Design and development of robust, cell-based, aging- and age-associated disease-relevant assays suitable for high-throughput screening of whole-genome single-guide (sg) RNA CRISPR libraries (human and mouse)
- Performance of high-throughput CRISPR whole-genome loss-of-function screens using human and mouse-arrayed sgRNA CRISPR libraries
- Bioinformatic analyses, prioritization of gene candidates, and validation of genes identified
Core Leader
Matthew J. Hart, PhD
Email